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Seminar: Paul Chadderton
November 15, 2018 @ 16:00 - 17:00
Combining mGRASP and optogenetics enables high-resolution functional mapping of descending cortical projections
Thursday 15 November – 4pm at the Large Lecture Theatre, DPAG/Le Gros Clarke Bldg
The Cortex Club is excited to host Dr Paul Chadderton from the University of Bristol, who will be talking to us about novel approaches to map both morphology and strength of synaptic connections. Please join us on November 15th at the Large Lecture Theatre, located in the Le Gros Clark Building of the Department of Physiology, Anatomy and Genetics.
Dr Paul Chadderton has kindly agreed to meet students and staff individually. If you would like to arrange a meeting please contact Lukas Krone (lukas.krone -at- dpag.ox.ac.uk).
To join us at the pub after the talk please register at https://goo.gl/forms/S4IqLbhLIs4ri3j93
Detailed mapping of neuronal connectivity is essential to understand the function of
brain circuitry. At the cellular and circuit level, this corresponds to precise information
about the number, location and strength of synaptic inputs received onto individual
neurons. Several methods have been developed to map the anatomical distribution
of synapses across neurons, but these approaches do not provide information about
the strength of individual connections. It is therefore difficult to confirm the functional
influence of connections and circuit mapping remains incomplete. In this talk, I will
describe a new approach to infer the strength of descending cortical synapses using
the anatomical marker, mGRASP (mammalian GFP reconstituted across synaptic
partners), a light microscopy technique that labels synaptic contacts. By
combining optogenetics and mGRASP, we have mapped the number and strength of
defined corticocollicular connections and reveal synaptic weighting of defined
projections at the level of single neurons. Overall these data demonstrate how subtle
variations in the functional organisation of cortical synapses influence overall circuit